Purification, Characterization and Serological Properties of a Glycolipid Antigen Reactive with a Serovar-specific Monoclonal Antibody against Leptospira Interrogans serovar Canicola
نویسندگان
چکیده
منابع مشابه
Evaluation of recombinant Leptospira interrogans serovar canicola outer membrane proteins as diagnostic antigen.
Leptospirosis of animals and man is an important rereported in recent years are LipL32, LipL41 and OmpL1; they emerging infectious disease worldwide. In domestic animals are expressed in-vivo during infection. LipL32 and LipL41 it is an important cause of abortion, stillbirth, infertility, are important and surface-exposed leptospiral outer membrane decreased milk production and death. In dairy...
متن کاملIsolation and Characterization of Two Novel Plasmids from Pathogenic Leptospira interrogans Serogroup Canicola Serovar Canicola Strain Gui44
BACKGROUND Previous genomic analysis of pathogenic Leptospira has identified two circular chromosomes but no plasmid. This study aims to investigate potential extrachromosomal elements of L.interrogans serovar Canicola strain Gui44. METHODOLOGY Two novel plasmids, pGui1 and pGui2, were isolated from the pathogenic strain Gui44, using a modified alkaline lysis method. Southern blotting was per...
متن کاملImmunological characteristics of the glycolipid antigen of Leptospira interrogans serovar lai.
The protective antigen (PAg), a glycolipid substance, was extracted from Leptospira interrogans serovar lai strain 017 with a chloroform-methanol-water (1:2:0.8 [vol/vol/vol]) solution and partially purified by silica gel column chromatography. The PAg was not detected by Coomassie brilliant blue staining in sodium dodecyl sulfate-polyacrylamide gel electrophoresis but was observed as a smearli...
متن کاملCharacterisation of the Proteome of Leptospira interrogans Serovar Canicola as a Resource for the Identification of Common Serovar Immunogenic Proteins
Over 230 serovars of Leptospira interrogans have been identified; however few have been completely characterised. The aim of this study was to characterise the proteome of serovar Canicola and to compare this against the serovars of Copenhageni and Pomona. 2D-LC/MS analysis identified 1653 Leptospira proteins in serovar Canicola; 60 of these proteins were common to Copenhageni and Pomona, 16 of...
متن کاملA neutral sugar is responsible for serovar specificity of the antigenic determinant of Leptospira interrogans serovar canicola.
To provide information on the chemical structures of antigenic determinants of leptospira, glycolipids of Leptospira interrogans serovar canicola strain Hond Utrecht IV (Ut-IV) and its antigenic variant selected in the presence of a serovar-specific monoclonal antibody were compared physicochemically. Gas-liquid chromatography-mass spectrometry analysis revealed that the glycolipid of Ut-IV con...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1987
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-133-5-1329